Recombinant DNA Labs: Basic Tools For The Molecular Biologist

Teachers Guide: Plasmid Isolation

Learner Outcomes

The following cognitive objectives should be addressed:

  1. Define vocabulary related to molecular biology: plasmid, bacteria, alkali, aseptic, lyse, DNA, RNA, precipitate, and isotonic.
  2. Explain or discuss the use of bacteria and plasmids in studying human genes.
  3. Explain how plasmids are beneficial to bacteria.
  4. Explain why scientists insert genes into plasmids.
The following laboratory objectives should be addressed. The student will be able to:
  1. Use aseptic techniques in transferring bacterial colonies to bacterial growth media.
  2. Pipette reagents.
  3. Separate liquid (supernatant) from a pellet (precipitate) by pipetting, after centrifugation.

 

 

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