PCR Learner Outcomes

The following cognitive objectives should be addressed. The student will be able to:

  1. Define the vocabulary related to PCR: DNA, Taq polymerase, primer, denature, anneal, elongate, target sequence.
  2. Describe the work of Kary Mullis as it relates to PCR.
  3. State why PCR is used to identify many bacteria and viruses.
  4. Relate genetic constitution of an organism with its physical attributes: describe the relationship between genotype and phenotype.
  5. Apply the technology used for PCR to current work in forensics and genetic testing.
  6. Discuss the moral and ethical issues of determining a person's genotype.

The following laboratory objectives should be addressed. The student will be able to:

  1. Pipette reagents.
  2. Wash a pelletized cell sample.
  3. Load and run a gel for electrophoresis.
  4. Compare experimental data with accepted values.